EP Challenge Test

European Pharmacopoeia - 5.1.3 “Efficacy of Antimicrobial Preservation"

This Preservative Efficacy Test (PET) or cosmetic challenge test, is essential for assessing the safety of cosmetic products susceptible to spoiling, such as those which require preservation or that contain water. According to the EU Cosmetic Products Regulation, for these cosmetic products it is a legal requirement to include the results of a challenge test in the Cosmetic Product Safety Report (CPSR) before they can be sold.

The European Pharmacopoeia test method is commonly used for products sold in the European Union and United Kingdom but is recognised worldwide.

Which EP Test?

How Long?

Test Procedure

EP USP ISO
UK
EU
USA
World

EP, USP or ISO standard PET test?

EP USP ISO
Incubation (days) 28 28 28
Sample required (g) 250 250 250
Total Species 4/5 5 5
Staphylococcus aureus
Pseudomonas aeruginosa
Candida albicans
Aspergillus brasiliensis
Escherichia coli

PET test details

European Pharmacopoeia (EP)